FLUOROMETRIC ASSAY FOR PLASMA PREKALLIKREIN USING PEPTIDYLMETHYL-COUMARINYL-AMIDE AS A SUBSTRATE

被引：34

作者：

OHISHI, S

KATORI, M

机构：

[1] Department of Pharmacology, Kitasato University School of Medicine, Sagamihara, Kanagawa

来源：

THROMBOSIS RESEARCH | 1979年 / 14卷 / 4-5期

关键词：

D O I：

10.1016/0049-3848(79)90111-7

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

The content of prekallikrein in human plasma was determined by activation with acetone and kaolin, and subsequent measurement of the enzyme activity using carbobenzoxy-L-phenylalanyl-L-arginine 4-methylcoumarinyl-7-amide as substrate. The kallikrein generated was stable for 60 min after activation and at least 10% of the original concentration of both Hageman factor and high molecular weight kininogen are required to get complete activation of prekallikrein within this time period. When this method was compared to the radiochemical method employing 3H-TAME a similar sensitivity could be demonstrated. Plasmin and hog pancreatic kallikrein less readily hydrolyzed the amide substrate. © 1979.

引用

页码：551 / 559

页数：9

共 12 条

[1]

Colman R W, 1976, Methods Enzymol, V45, P303

[2] WILLIAMS TRAIT - HUMAN KININOGEN DEFICIENCY WITH DIMINISHED LEVELS OF PLASMINOGEN PROACTIVATOR AND PREKALLIKREIN ASSOCIATED WITH ABNORMALITIES OF HAGEMAN FACTOR-DEPENDENT PATHWAYS [J].