BIOACCUMULATION OF PCDDS AND PCDFS IN FOOD ANIMALS .3. A RAPID CLEANUP OF BIOLOGICAL-MATERIALS USING REVERSE PHASE ADSORBENT COLUMNS

This study describes a simple, rapid and cost effective analytical technique for the ultratrace analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F) in biological samples. Solid Phase Extraction (SPE) techniques using disposable C-18 bonded silica column for dioxin enrichment have been investigated. The procedures used for extraction of PCDD/F from animal tissues and blood plasma are described. Spiked recoveries of surrogates from eggs, livers, birds and blood plasma are reported. The suitability of SPE for the determination of PCDD/F is demonstrated by the analysis of contaminated biological samples. Comparison of results obtained from the SPE method and from the traditional sulfuric acid cleanup is presented. © 1990.