A RAPID METHOD FOR THE ISOLATION OF GENOMIC DNA FROM CITRATED WHOLE-BLOOD

被引：45

作者：

PARZER, S

MANNHALTER, C

机构：

来源：

BIOCHEMICAL JOURNAL | 1991年 / 273卷

关键词：

D O I：

10.1042/bj2730229

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a DNA isolation method which allows the isolation of high quality DNA from frozen citrated blood and cell lines within 90 min. The procedure avoids the use of toxic organic reagents and tedious extraction steps. DNA prepared by this method is very well suited for restriction fragment length polymorphism and polymerase chain reaction analysis.

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页码：229 / 231

页数：3

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