BIOSENSOR FOR CATECHOLAMINES WITH IMMOBILIZED MONOAMINE-OXIDASE IN TISSUE-SECTIONS

被引：12

作者：

BUDANTSEV, AY

机构：

[1] Institute of Theoretical and Experimental Biophysics, U.S.S.R. Academy of Sciences, Pushchino

来源：

ANALYTICA CHIMICA ACTA | 1991年 / 249卷 / 01期

关键词：

VOLTAMMETRY; BIOSENSORS; CATECHOLAMINES; ENZYME REACTOR; INDOLEAMINES; ION-SELECTIVE ELECTRODES;

D O I：

10.1016/0003-2670(91)87010-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of a tissue-based biosensor for the measurement of the concentration of catecholamines and indolamines is described. The bioelement of the biosensor is a rat liver section and the reaction of the deamination of biogenic amines (monoamineoxidase) was used. The concentration of NH4+ was measured by using an ammonium-selective electrode. The biosensor was used (OT measuring tyramine, dopamine, tryptamine and serotonin in the range ca. 10(-3)-10(-4) M; the lifetime of the biosensor is about 20-25 days. Optimization of the parameters of the biosensor is discussed.

引用

页码：71 / 76

页数：6

共 34 条

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