LEUKOCYTE-DEPLETED PLATELETS PREPARED FROM POOLED BUFFY COAT POSTTRANSFUSION INCREMENT AND INVITRO BLEEDING-TIME USING THE THROMBOSTAT 4000/2

被引：7

作者：

HOGMAN, CF

ERIKSSON, L

KRISTENSEN, J

机构：

[1] Department of Clinical Immunology and Transfusion Medicine

[2] Department of Haematology, University Hospital

来源：

TRANSFUSION SCIENCE | 1993年 / 14卷 / 01期

关键词：

D O I：

10.1016/0955-3886(93)90051-U

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A technique for preparation of platelet concentrate (PC) from pooled buffy coat (BC) is described in this study. The yield of platelets from 4 BCs was 310+/-48 X 10(9) per unit with a leukocyte content of 21+/-20 X 10(6) per unit. When a leukocyte removing filter was interconnected, Sepacell 5N and Pall PL-50 filters, no leukocytes could be found in 56 of 59 and in 36 of 37 PCs, with a maximum content of 2.3 and 0.54 X 10(6) leukocytes per PC, respectively. The platelet yield was 276+/-37 and 279+/-54 X 10(9) per unit, respectively. The in vitro bleeding time (IVBT), using the Thrombostat 4000/2, corresponded with the corrected count increment in most of 11 thrombocytopenic patients receiving platelet transfusions. However, in two transfusions no correction of IVBT was obtained. This technique offers a new possibility to investigate the functional capacity of transfused platelets.

引用

页码：35 / 39

页数：5

共 5 条

[1]

Eriksson, Hogman, Platelet concentrates in an additive solution prepared from pooled buffy coats. 1. In vitro studies, Vox Sang, 59, pp. 140-145, (1990)

[2]

Kratzer, Born, Simulation of primary haemostasis in vitro, Haemostasis, 15, pp. 357-362, (1985)

[3]

Kretschmer, Schikor, Sohngen, Dietrich, In vitro bleeding test—a simple method for the sensitive detection of the aspirin effect on platelet function, Thromb Res, 56, pp. 593-602, (1989)

[4]

Hogman, Eriksson, Ring, Automated blood component preparation with the Opti System: three years experience, Infusionstherapie, (1993)

[5]

Hogman, Eriksson, Gulliksson, Shanwell, Preparation of platelet concentrate from pooled buffy coats suspended in an additive solution, Transfusion Science, 11, pp. 353-355, (1990)

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