DETECTION OF 47,XYY TROPHOBLAST FETAL CELLS IN MATERNAL BLOOD BY FLUORESCENCE INSITU HYBRIDIZATION AFTER USING IMMUNOMAGNETIC LYMPHOCYTE DEPLETION AND FLOW-CYTOMETRY SORTING

被引：52

作者：

CACHEUX, V

MILESIFLUET, C

TACHDJIAN, G

DRUART, L

BRUCH, JF

HSI, BL

UZAN, S

NESSMANN, C

机构：

[1] Laboraloire de Biologie du Développement el de la Reproduction, Hópital Robert-Debre, Paris

[2] Générale de Recherche Médicale S A, Paris

[3] Service de Gynécologic-Obstétrique, Hôpital Tenon, Paris

来源：

FETAL DIAGNOSIS AND THERAPY | 1992年 / 7卷 / 3-4期

关键词：

MATERNAL BLOOD SAMPLING; TROPHOBLAST FETAL CELLS; PRENATAL DIAGNOSIS; FLOW CYTOMETRY SORTING; FLUORESCENCE INSITU HYBRIDIZATION; 47; XYY KARYOTYPE;

D O I：

10.1159/000263699

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Fluorescence in situ hybridization (FISH) allows to diagnose aneuploidy in uncultured interphase nuclei. This rapid method of chromosomal analysis associated with cell-sorting techniques was realized on 47,XYY fetal cells isolated from maternal blood. Trophoblast cells were sorted by combining immunomagnetic removal of maternal lymphocytes and flow cytometry sorting using antitrophoblast monoclonal antibodies. Cells were sorted directly on slides and analyzed by FISH with a Y-centromeric probe. Among 1,387 examinable nuclei, 59 (4.25%) showed one single or two Y-specific domains.

引用

页码：190 / 194

页数：5

共 20 条

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