REGULATION OF CUCUMBER CLASS-III CHITINASE GENE-EXPRESSION

被引：55

作者：

LAWTON, KA ^{[1
]}

BECK, J ^{[1
]}

POTTER, S ^{[1
]}

WARD, E ^{[1
]}

RYALS, J ^{[1
]}

机构：

[1] CIBA AGR BIOTECHNOL,DEPT MOLEC GENET,RES TRIANGLE PK,NC 27709

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1994年 / 7卷 / 01期

关键词：

SYSTEMIC ACQUIRED RESISTANCE;

D O I：

10.1094/MPMI-7-0048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The chromosomal region encoding the acidic class III chitinase from cucumber has been isolated and characterized. As a result of an apparent gene triplication, the pathogen-induced gene (CHI2) is flanked by two closely related genes with complete open reading frames (ORF). The high level of conservation within the three ORFs suggests an essential role for each encoded protein in plant growth and development. The developmental and tissue-specific expression of RNA from each gene was analyzed using both gene-specific probes and RNA-PCR. The expression of each gene in response to various inducing treatments was also characterized. Only transcripts corresponding to CHI2 were detected. Chitinase mRNA abundance increased slightly following cycloheximide application; however, its potent induction by salicylic acid was inhibited by cycloheximide treatment.

引用

页码：48 / 57

页数：10

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