NADH COUPLED ASSAY SYSTEM FOR GALACTOSE OXIDASE

被引：9

作者：

AVIGAD, G

机构：

[1] Department of Biochemistry, College of Medicine and Dentistry of New Jersey, Rutgers Medical School, Piscataway

来源：

ANALYTICAL BIOCHEMISTRY | 1978年 / 86卷 / 02期

关键词：

D O I：

10.1016/0003-2697(78)90771-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A galactose oxidase (EC 1.1.3.9); NADH-peroxidase (EC 1.11.1.1) coupled assay system is used for the estimation of galactose oxidase activity. Spectrophotometric measurement of NADH consumption yields direct quantitative value of enzymic activity or can be used for the end-point determination of the amount of galactose oxidase substrate present in test solutions. Use of similar coupled systems is suggested for the assay of other H2O2-producing enzymes and their substrates. © 1978.

引用

页码：470 / 476

页数：7

共 28 条

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