PHYTOALEXIN PRODUCTION BY LEAVES OF PISUM SATIVUM IN RELATION TO SENESCENCE

A sterile culture nitrate of Penicillium expansum was shown to induce pisatin synthesis in pea leaf discs. The amount of pisatin produced by pea leaves was shown to decrease as they underwent senescence. N6‐benzyladenine delayed senescence, and at the same time maintained the production of pisatin at a high level. In darkness, leaf discs maintained on either benzyl‐adenine solution or distilled water produced greater amounts of pisatin than leaf discs which were not treated in this way. Benzyladenine also increased pisatin production by leaf discs kept in the light. The relevance of these results to disease resistance and possible mechanisms involved are discussed. Copyright © 1969, Wiley Blackwell. All rights reserved