ANTIGENICITY OF HUMAN HEMOGLOBINS .I. THE ANTIGENICITY OF HEMOGLOBIN SUBUNIT CHAINS DEMONSTRATED BY UREA ADDED IMMUNOELECTROPHORESIS

By double diffusion and cellulose acetate membrane immunoelectrophoresis the complexity of the Hb-A, Hb-F antigens and anti-Hb-A antiserum was demonstrated though the anti-Hb-F antiserum contained only 1 antibody against Hb-F. The urea added immunoelectrophoresis which consists of the electrophoretical separation of subunit chains on the cellulose acetate membrane under the existence of 8 m urea and the application of antiserum after the removal of urea was used to investigate the antigenicity of hemoglobin subunit chains. In spite of the complexity of system used, 2 lines, for the fast moving α-chain and for the slow moving β-chain, were demonstrated in anti-Hb-A antiserum and Hb-A system. Also the α-chain line in anti-Hb-A antiserum and Hb-F system and the γ-chain line in anti-Hb-F antiserum and Hb-F system were demonstrated. © 1969 J. F. Lehmanns Verlag.