SIMPLE RAPID METHOD FOR GANGLIOSIDE ISOLATION FROM SMALL AMOUNTS OF TISSUE

被引：80

作者：

IRWIN, CC

IRWIN, LN

机构：

[1] Eunice Kennedy Shriver Center for Mental Retardation, Waltham

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/0003-2697(79)90369-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gangliosides from as little as 1 mg dry wt of brain tissue can be isolated for thin-layer chromatography by a simple, rapid method which combines extraction by chloroformmethanol with a single step silicic acid column separation of gangliosides from the bulk of nonganglioside lipids. © 1979.

引用

页码：335 / 339

页数：5

共 19 条

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[J]. ANALYTICAL BIOCHEMISTRY, 1978, 86 (02) : 543 - 551
[7] INCORPORATION OF RADIOACTIVITY OF D-GLUCOSAMINE-1-C-14 INTO HETEROPOLYSACCHARIDE CHAINS OF GLYCOPROTEINS IN ADULT AND DEVELOPING RAT BRAIN
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[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1971, 142 (01) : 111 - +
[8] EFFECT OF HYPERCAPNIA IN VIVO ON RAT BRAIN GANGLIOSIDES
HOLM, MR
[J]. JOURNAL OF NEUROCHEMISTRY, 1968, 15 (08) : 821 - &
[9] IRWIN C, 1978, ABSTR SOC NEUR, V4, P116
[10] IRWIN CC, 1978, FED PROC, V37, P1645

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