25-HYDROXYLATION OF VITAMIN-D3 BY A RECONSTITUTED SYSTEM FROM RAT-LIVER MICROSOMES

被引：24

作者：

BJORKHEM, I

HANSSON, R

HOLMBERG, I

WIKVALL, K

机构：

[1] UNIV UPPSALA, DEPT PHARMACEUT BIOCHEM, S-75105 UPPSALA, SWEDEN

[2] KAROLINSKA INST, HUDDINGE HOSP, CTR RES, S-10401 STOCKHOLM 60, SWEDEN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 90卷 / 02期

关键词：

D O I：

10.1016/0006-291X(79)91279-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using isotope dilution-mass fragmentography as assay technique, it was shown that highly purified preparations of cytochrome P-450 from rat liver microsomes catalyzed 25-hydroxylation of vitamin D3 when combined with NADPH-cytochrome P-450 reductase and a phospholipid. The rate of conversion was approximately linear with the amount of cytochrome P-450, and was considerably higher than the rate of conversion obtained with crude liver microsomes. The possibility is discussed that the microsomal fraction contains inhibitors of 25-hydroxylase activity, which may be of regulatory importance in vitamin D3 metabolism. © 1979.

引用

页码：615 / 622

页数：8

共 21 条

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[10] PROPERTIES OF RECONSTITUTED CHOLESTEROL 7 ALPHA-HYDROXYLASE SYSTEM FROM RAT AND RABBIT LIVER-MICROSOMES
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