ADVENTITIOUS SHOOT FORMATION IN CULTURES OF 30-YEAR-OLD LARIX-DECIDUA, L-LEPTOLEPIS, L-EUROLEPIS, AND L-LARICINA TREES

被引:23
作者
BONGA, JM
POND, SE
机构
[1] Forestry Canada, Maritimes Region, Fredericton, E3B 5P7, N.B.
关键词
LARCH; LARIX-DECIDUA; L-EUROLEPIS; L-LARICINA; L-LEPTOLEPIS; MATURE TREES; MICROPROPAGATION;
D O I
10.1007/BF00116609
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Primordial shoot explants excised from buds of one Larix decidua tree, about 30 years old, produced more adventitious buds, elongating into shoots, when grown on half strength Litvay medium than when grown on other basal media. Thidiazuron and N6-benzyladenine (BA) were equally effective in adventitious bud induction. In a comparative study of 30-year-old L. decidua, L. leptolepis, L. eurolepis, and L. laricina trees, explants from L. eurolepis and L. decidua produced a high number of cultures with adventitious buds that elongated into shoots; those from L. leptolepis were less productive, and those from L. laricina failed to form adventitious buds. The highest response was obtained with material collected in August and September, and in March and April; the lowest response occurred in explants from the October collection.
引用
收藏
页码:45 / 51
页数:7
相关论文
共 15 条
[1]  
BEKKAOUI F, 1986, ANN RECHERCHES SYLVI, P63
[2]  
Bonga J. M., 1988, Somatic cell genetics of woody plants. Proc. IUFRO Working Party S2.04-07, held in Grosshansdorf, W. Germany, 10-13 Aug. 1987., P155
[3]  
BONGA JM, 1991, CLONAL FORESTRY GENE
[4]  
Chalupa V., 1985, Communicationes Instituti Forestalis Cechosloveniae, V14, P65
[6]  
CHALUPA V, 1987, L BIOL PLANT, V29, P425
[7]   INVITRO MULTIPLE BUD FORMATION BY 20-YEAR-OLD WESTERN LARCH BUDS AND STEMS [J].
CHESICK, EE ;
BILDERBACK, DE ;
BLAKE, GM .
HORTSCIENCE, 1990, 25 (01) :114-116
[8]  
CHEVREAU E, 1989, PLANT CELL REP, V7, P688, DOI 10.1007/BF00272062
[9]  
DUMAS E, 1986, ANN AFOCEL, P95
[10]   SUSTAINED CAULOGENESIS IN CALLUS-CULTURES OF LARIX X EUROLEPIS INITIATED FROM SHORT SHOOT BUDS OF A 12-YEAR-OLD TREE [J].
LALIBERTE, S ;
LALONDE, M .
AMERICAN JOURNAL OF BOTANY, 1988, 75 (06) :767-777