TC-99M-EXAMETAZIME-LABELED LEUKOCYTES - EFFECT OF VOLUME AND CONCENTRATION OF EXAMETAZIME ON LABELING EFFICIENCY, AND CLINICAL PROTOCOL FOR HIGH-EFFICIENCY MULTIDOSE RADIOLABELING

被引:19
作者
SAMPSON, CB
SOLANKI, C
BARBER, RW
机构
[1] Department of Nuclear Medicine, Addenbrooke's Hospital, Cambridge, Hills Road
关键词
D O I
10.1097/00006231-199108000-00007
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
We have investigated the effect of volume and concentration of exametazime on the labelling efficiency of Tc-99(m)-exametazime-labelled leucocytes. The first study examined the effect of varying the volume of exametazime solution whilst the concentration remained constant. A vial of Ceretec (Amersham Ltd plc) was reconstituted with sodium chloride injection BP. Aliquots of 0.25-2.0 ml were removed, added to sodium pertechnetate injection BP and incubated with 2 x 10(7)-5 x 10(7) leucocytes from 25 ml blood. The labelling efficiency decreased from 65 +/- 10% S.D. (0.25 ml) to 45 +/- 8% (2.0 ml) (n = 4). In a second study different concentrations of exametazime solution were used whilst the volume remained constant. A vial of Ceretec was reconstituted with sodium chloride injection BP. Aliquots of 25-200-mu-g were removed, made up to a fixed volume of 0.6 ml, added to pertechnetate and incubated with the plug of leucocytes as before. The labelling efficiency decreased as the concentration of ligand decreased. Thus for 200-mu-g/0.6 ml the labelling efficiency was 64 +/- 5% and for 25-mu-g/0.6 ml the labelling efficiency was 43 +/- 18% (n = 4). Clinical studies were performed using 50 ml blood from patients with a wide range of inflammatory disorders including inflammatory bowel disease, abdominal abscess and vasculitis. The concentration of ligand used was 83-mu-g/0.25 ml. The labelling efficiency was found to be 82 +/- 7% (n = 36).
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页码:719 / 723
页数:5
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