AUTOMATED-DETERMINATION OF OXYTETRACYCLINE RESIDUES IN MUSCLE, LIVER, MILK AND EGG BY ONLINE DIALYSIS AND POSTCOLUMN REACTION DETECTION HPLC

被引：22

作者：

AGASOSTER, T

机构：

[1] Institute of Pharmacy, University of Oslo, Oslo, N‐0316

来源：

FOOD ADDITIVES AND CONTAMINANTS | 1992年 / 9卷 / 06期

关键词：

OXYTETRACYCLINE; DIALYSIS; TISSUES; MILK; EGG;

D O I：

10.1080/02652039209374116

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An automated method for control of oxytetracycline (OTC) residues iri chicken and bovine muscle, salmon liver, bovine milk and hen egg has been developed. Tissue homogenate, decreamed milk or whole egg solution was dialysed and the dialysate enriched on a small polystyrene column on-line to HPLC. OTC and the internal standard (tetracycline) were separated on a polystyrene column by ion-pair chromatography. The column effluent was mixed with sodium hydroxide and irradiated at 366 nm. Monitoring the resulting derivatives with a fluorescence detector (excitation: 358 nm, emission: 460 nm), OTC could be detected at 1 ng/ml in milk, 1 ng/g in egg, 3-4 ng/g in muscle and 8 ng/g in liver. Relative standard deviations at 50 and 200 ng/g (milk: 20 and 100 ng/ml) ranged from 1.6 to 3.1%.

引用

页码：615 / 622

页数：8

共 18 条

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