TRANSCRIPTION AND TRANSLATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN THE PANCREAS OF PREDIABETIC BB RATS

被引：141

作者：

KLEEMANN, R

ROTHE, H

KOLBBACHOFEN, V

XIE, QW

NATHAN, C

MARTIN, S

KOLB, H

机构：

[1] UNIV DUSSELDORF,INST IMMUNOBIOL,D-40225 DUSSELDORF,GERMANY

[2] CORNELL UNIV,MED CTR,COLL MED,DEPT MED,NEW YORK,NY 10021

来源：

FEBS LETTERS | 1993年 / 328卷 / 1-2期

关键词：

DIABETES TYPE-1; BB RAT; INSULITIS; NO SYNTHASE; NITRIC OXIDE;

D O I：

10.1016/0014-5793(93)80954-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The inducible NO synthase (iNOS) was found to be expressed in pancreatic lesions of adult diabetes-prone BB rats. Pancreatic iNOS mRNA was detected by reverse transcriptase PCR in pancreatic RNA of adult diabetes-prone BB rats but not in normal Wistar rats, young diabetes-prone BB rats without insulitis or in diabetes-resistant BB rats. Immunohistochemistry of pancreatic sections using an iNOS-specific antiserum labeled the pancreas of adult diabetes-prone BB rats but not Wistar rats. Parallel staining for ED1-positive macrophages showed restriction of iNOS expression to areas of islet infiltration by macrophages. In conclusion, the data provide direct evidence for enhanced expression of inducible NO synthase in tissue lesions during the development of autoimmune diabetes.

引用

页码：9 / 12

页数：4

共 20 条

[1] CYTOTOXIC ACTION OF IL-1-BETA AGAINST PANCREATIC-ISLETS IS MEDIATED VIA NITRIC-OXIDE FORMATION AND IS INHIBITED BY N(G)-MONOMETHYL-L-ARGININE [J].