TRIPLE-HELIX FORMATION AT A8XA8.T8YT8

被引：23

作者：

CHANDLER, SP ^{[1
]}

FOX, KR ^{[1
]}

机构：

[1] UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,BASSETT CRESCENT E,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

TRIPLE HELIX; SEQUENCE RECOGNITION; STRINGENCY; DNASE-I FOOTPRINTING;

D O I：

10.1016/0014-5793(93)80510-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have examined the formation of DNA triple helices between the oligonucleotides T8XT8 (X = A,C,G,T) and DNA fragments containing thc target sequences A8XA8 . T8YT8 (X = T,C,G; Y = A,G,C), by DNase I footprinting. We find that A8GA8 . T8CT8 yields a footprint with T8CT8 and shows a weaker interaction with T17 and T8GT8 . A8CA8 . T8GT8 yields a footprint with T17, and shows weaker interaction with T8CT8 . A8TA8 . T8AT8 yields a footprint with T8GT8 and shows weaker interaction with T17. Each of the successful complexes is characterised by enhanced DNase I cleavage at the 3' end of the purine strand of the target, as well as protection at the 5' end- We have been unable to form triplexes with third strands of the type A8XA8.

引用

页码：189 / 192

页数：4

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