THE HUMAN AND MOUSE HOMOLOGS OF THE YEAST RAD52 GENE - CDNA CLONING, SEQUENCE-ANALYSIS, ASSIGNMENT TO HUMAN-CHROMOSOME 12P12.2-P13, AND MESSENGER-RNA EXPRESSION IN MOUSE-TISSUES

被引：65

作者：

SHEN, ZY ^{[1
]}

DENISON, K ^{[1
]}

LOBB, R ^{[1
]}

GATEWOOD, JM ^{[1
]}

CHEN, DJ ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB,DIV LIFE SCI,LOS ALAMOS,NM 87545

来源：

GENOMICS | 1995年 / 25卷 / 01期

关键词：

D O I：

10.1016/0888-7543(95)80126-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The yeast Saccharomyces cerevisiae RAD52 gene is involved in DNA double strand break repair and mitotic/meiotic recombination. The N-terminal amino acid sequence of yeast S. cerevisiae, Schizosaccharomyces pombe, and Kluyveromyces lactis and chicken is highly conserved. Using the technology of mixed oligo nucleotide primed amplification of cDNA (MOPAC), two mouse RAD52 homologous cDNA fragments were amplified and sequenced. Subsequently, we have cloned the cDNA of the human and mouse homologs of yeast RAD52 gene by screening cDNA libraries using the identified mouse cDNA fragments. Sequence analysis of cDNA derived amino acid revealed a highly conserved N-terminus among human, mouse, chicken, and yeast RAD52 genes. The human RAD52 gene was assigned to chromosome 12p12.2-p13 by fluorescence in situ hybridization, R-banding, and DNA analysis of somatic cell hybrids. Unlike chicken RAD52 and mouse RAD51, no significant difference in mouse RAD52 mRNA level was found among mouse heart, brain, spleen, lung, liver, skeletal muscle, kidney, and testis. In addition to an similar to 1.9-kb RAD52 mRNA band that is present in all of the tested tissues, an extra mRNA species of similar to 0.85 kb was detectable in mouse testis. (C) 1995 Academic Press, Inc.

引用

页码：199 / 206

页数：8

共 40 条

[1] PRIMARY STRUCTURE OF THE RAD52 GENE IN SACCHAROMYCES-CEREVISIAE [J].