IMMOBILIZATION OF CITRATE-PRODUCING YARROWIA-LIPOLYTICA CELLS IN POLYELECTROLYTE COMPLEX CAPSULES

被引：17

作者：

FORSTER, M

MANSFIELD, J

SCHELLENBERGER, A

DAUTZENBERG, H

机构：

[1] UNIV HALLE WITTENBERG, INST BIOCHEM, DEPT BIOCHEM & BIOTECHNOL, D-06120 HALLE, GERMANY

[2] UNIV POTSDAM, DEPT CHEM, POLYELECTROLYTE COMPLEXES RES GRP, TELTOW, GERMANY

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 09期

关键词：

IMMOBILIZATION; ENCAPSULATION; POLYELECTROLYTE COMPLEX MICROCAPSULES; POLY(DIMETHYLDIALLYLAMMONIUM CHLORIDE); CELLULOSE SULFATE; CITRATE-PRODUCING MICROORGANISMS; YARROWIA LIPOLYTICA; FLUIDIZED BED FERMENTATION;

D O I：

10.1016/0141-0229(94)90035-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microcapsules prepared from sodium cellulose sulfate and poly(dimethyldiallylammonium chloride) have been shown to be well suited for encapsulation of biological objects. The citrate-accumulating yeast Yarrowia lipolytica was immobilized using this method without severe lass of vitality and catalytic activity. The encapsulated cells had an average productivity of 0.125gl(-1) h(-1). The yield was 0.45g acid g(-1) glucose. I;he immobilized biocatalyst lost the catalytic activity, but was still able to divide after 2,000 h use.

引用

页码：777 / 784

页数：8

共 28 条

[1] BIOCATALYSIS WITH IMMOBILIZED CELLS [J].