AVIDIN MONOMER AFFINITY COLUMN FOR THE PURIFICATION OF BIOTIN-CONTAINING ENZYMES

被引：101

作者：

HENRIKSON, KP ^{[1
]}

ALLEN, SHG ^{[1
]}

MALOY, WL ^{[1
]}

机构：

[1] CASE WESTERN RESERVE UNIV,SCH MED,DEPT BIOCHEM,CLEVELAND,OH 44106

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90374-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure is given for the preparation of an avidin monomer affinity column which is useful in the purification of biotin-containing enzymes. Both the propionyl-CoA carboxylase of Mycobacterium smegmatis and the methylmalonyl-CoA pyruvate transcarboxylase (EC 2.1.3.1) of Arachnia propionica and Propionibacterium shermanii bind to the column and can be specifically eluted with (+)-biotin. © 1979.

引用

页码：366 / 370

页数：5

共 12 条

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[3] SEPHAROSE-AVIDIN COLUMN FOR BINDING OF BIOTIN OR BIOTIN-CONTAINING PEPTIDES
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[J]. EXPERIENTIA, 1970, 26 (03): : 327 - +
[4] DEMONSTRATION OF PHOSPHORYLATION OF ACETYL-COENZYME-A CARBOXYLASE WITH INTACT RAT EPIDIDYMAL FAT-CELLS
BROWNSEY, RW
HUGHES, WA
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[J]. BIOCHEMICAL JOURNAL, 1977, 168 (03) : 441 - 445
[5] GREEN N. M., 1970, Methods in Enzymology, V18A, P418, DOI 10.1016/0076-6879(71)18342-5
[6] PROPERTIES OF SUBUNITS OF AVIDIN COUPLED TO SEPHAROSE
GREEN, NM
TOMS, EJ
[J]. BIOCHEMICAL JOURNAL, 1973, 133 (04) : 687 - 698
[7] GUCHHAIT RB, 1974, J BIOL CHEM, V249, P6633
[8] HENRIKSON KP, 1978, FED PROC, V37, P1525
[9] MALOY WL, 1977, FED PROC, V36, P873
[10] MOSS J, 1971, ADV ENZYMOL RAMB, V35, P321

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