INDUCTION AND CULTIVATION OF A STABLE L-FORM OF BACILLUS-SUBTILIS

被引:25
作者
ALLAN, EJ
机构
[1] Division of Bacteriology, Department of Agriculture, University, Aberdeen
来源
JOURNAL OF APPLIED BACTERIOLOGY | 1991年 / 70卷 / 04期
关键词
D O I
10.1111/j.1365-2672.1991.tb02946.x
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The induction of L-forms of Bacillus subtilis from protoplasts is described. The method involved the frequent subculture of the unstable L-form on a growth medium supplemented with lysozyme and horse serum. A stable culture, which did not revert when lysozyme and horse serum were omitted from the medium, was obtained after 13 subcultures. This culture could be grown on solid and in liquid medium by routine microbiological methods. Long-term storage of these cells was achieved by freeze drying and maintenance in glycerol at -70-degrees-C. The cultural adaptability of the L-form is described and discussed with respect to methods of cultivation and growth.
引用
收藏
页码:339 / 343
页数:5
相关论文
共 13 条
[1]   A METHOD FOR THE CULTIVATION OF L-FORMS IN LIQUID MEDIA [J].
ABRAMS, RY .
JOURNAL OF BACTERIOLOGY, 1955, 70 (02) :251-251
[2]   ARTIFICIALLY INDUCED SYMBIOTIC ASSOCIATIONS OF L-FORM BACTERIA AND PLANTS [J].
ALOYSIUS, SKD ;
PATON, AM .
JOURNAL OF APPLIED BACTERIOLOGY, 1984, 56 (03) :465-477
[3]   INDUCTION AND PROPAGATION OF A BACILLUS SUBTILIS L FORM IN NATURAL AND SYNTHETIC MEDIA [J].
BURMEISTER, HR ;
HESSELTINE, CW .
JOURNAL OF BACTERIOLOGY, 1968, 95 (05) :1857-+
[4]  
GILPIN RW, 1986, BACTERIAL L FORMS, P99
[5]   L-PHASE OF ERWINIA-CAROTOVORA VAR ATROSEPTICA AND ITS POSSIBLE ASSOCIATION WITH PLANT-TISSUE [J].
JONES, SM ;
PATON, AM .
JOURNAL OF APPLIED BACTERIOLOGY, 1973, 36 (04) :729-&
[7]   GELATIN-INDUCED REVERSION OF PROTOPLASTS OF BACILLUS SUBTILIS TO BACILLARY FORM - BIOSYNTHESIS OF MACROMOLECULES AND WALL DURING SUCCESSIVE STEPS [J].
LANDMAN, OE ;
FORMAN, A .
JOURNAL OF BACTERIOLOGY, 1969, 99 (02) :576-&
[8]  
LANDMAN OE, 1977, MICROBIOLOGY 1977, P35
[9]  
LAPAGE SP, 1970, METHODS MICROBIOLO A, V3, P136
[10]  
MADOFF S, 1976, RAPID DIAGNOSTIC MET, P195