MICHAELIS-MENTEN KINETICS OF NITRATE UPTAKE IN JAPANESE BARLEY CULTIVARS

被引：1

作者：

HASEGAWA, H

机构：

[1] Research Institute for Advanced Science and Technology, University of Osaka Prefecture

来源：

JAPANESE JOURNAL OF CROP SCIENCE | 1992年 / 61卷 / 02期

关键词：

BARLEY; MICHAELIS-MENTEN KINETICS; NITRATE UPTAKE; SEEDLING; TEMPERATURE;

D O I：

10.1626/jcs.61.251

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Nitrate uptake ability from low-concentration nitrate solution (below 1 mM) was evaluated based on Michaelis-Menten kinetics in 12 Japanese barley cultivars. Two kinetic parameters, Km and Vmax, were determined using 7-day-old seedlings that had been cultured with distilled water for 6 days and treated with induction medium (100-mu-M KNO3 plus 500-mu-M CaSO4) for 24h prior to the experiment. The uptake solution was composed of 25- 1 000-mu-M KNO3 and 500-mu-M CaSO4. At 25-degrees-C, Km and Vmax in the 12 cultivars used in this experiment ranged from 50.9 to 111.7-mu-M and from 2.24 to 4.99-mu-mol . g-1 f.w. . h-1, respectively. Km obtained in this experiment was roughly coincident with the values demonstrated by earlier studies in some plants including barley. A significantly high correlationship (r=0.896) was found between Km and Vmax. Km and Vmax decreased at low temperatures and the extent of the decrease in Km depended on the cultivars. The ratio of Km at 15-degrees-C to that at 25-degrees-C ranged from about 1.00 to less than 0.50. Furthermore, it was observed that Vmax was not related to such morphological traits as plant fresh weight, first leaf length, and root length.

引用

页码：251 / 256

页数：6

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