MITOTIC REPRESSION OF RNA POLYMERASE-III TRANSCRIPTION IN-VITRO MEDIATED BY PHOSPHORYLATION OF A TFIIIB COMPONENT

被引：121

作者：

GOTTESFELD, JM ^{[1
]}

WOLF, VJ ^{[1
]}

DANG, T ^{[1
]}

FORBES, DJ ^{[1
]}

HARTL, P ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO, DEPT BIOL, LA JOLLA, CA 92093 USA

来源：

SCIENCE | 1994年 / 263卷 / 5143期

关键词：

D O I：

10.1126/science.8272869

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interphase cytosol extracts prepared from Xenopus laevis eggs are active in RNA polymerase III (Pol III) transcription. Addition of recombinant B1 cyclin to these extracts activates mitotic protein kinases that repress transcription. Affinity-purified p34cdc2-cyclin B kinase (mitosis-promoting factor) is sufficient to effect this repression in a simplified Pol III transcription system. This mitotic repression involves the direct phosphorylation of a component of the Pol III transcription initiation factor TFIIIB, which consists of the TATA box-binding protein (TBP) and associated Pol III-specific factors. The transcriptional activity of the TFIIIB-TBP fraction can be modulated in vitro by phosphorylation with mitotic kinases and by dephosphorylation with immobilized alkaline phosphatase.

引用

页码：81 / 84

页数：4

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