INTRODUCTION OF ARBITRARY SEQUENCES INTO GENES BY USE OF CLASS-IIS RESTRICTION ENZYMES

被引：6

作者：

BECK, R ^{[1
]}

BURTSCHER, H ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM RES CTR,POSTFACH 1152,D-82372 PENZBERG,GERMANY

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 05期

关键词：

D O I：

10.1093/nar/22.5.886

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：886 / 887

页数：2

共 6 条

[1]

BECK R, 1994, IN PRESS PROTEIN EXP

[2]

Ford C F, 1991, Protein Expr Purif, V2, P95, DOI 10.1016/1046-5928(91)90057-P

[3] SITE-DIRECTED MUTAGENESIS BY OVERLAP EXTENSION USING THE POLYMERASE CHAIN-REACTION [J].

HO, SN ;

HUNT, HD ;

HORTON, RM ;

PULLEN, JK ;

PEASE, LR .

GENE, 1989, 77 (01) :51-59

[4] ENGINEERING HYBRID GENES WITHOUT THE USE OF RESTRICTION ENZYMES - GENE-SPLICING BY OVERLAP EXTENSION [J].

HORTON, RM ;

HUNT, HD ;

HO, SN ;

PULLEN, JK ;

PEASE, LR .

GENE, 1989, 77 (01) :61-68

[5] A SIMPLE AND RAPID METHOD FOR GENERATING A DELETION BY PCR [J].

IMAI, Y ;

MATSUSHIMA, Y ;

SUGIMURA, T ;

TERADA, M .

NUCLEIC ACIDS RESEARCH, 1991, 19 (10) :2785-2785

[6] CLASS-IIS RESTRICTION ENZYMES - A REVIEW [J].

SZYBALSKI, W ;

KIM, SC ;

HASAN, N ;

PODHAJSKA, AJ .

GENE, 1991, 100 :13-26

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