URINARY-EXCRETION OF PODOCALYXIN INDICATES GLOMERULAR EPITHELIAL-CELL INJURIES IN GLOMERULONEPHRITIS

被引：90

作者：

HARA, M ^{[1
]}

YAMAMOTO, T ^{[1
]}

YANAGIHARA, T ^{[1
]}

TAKADA, T ^{[1
]}

ITOH, M ^{[1
]}

ADACHI, Y ^{[1
]}

YOSHIZUMI, A ^{[1
]}

KAWASAKI, K ^{[1
]}

KIHARA, I ^{[1
]}

机构：

[1] NIIGATA UNIV,SCH MED,INST NEPHROL,DEPT PATHOL,NIIGATA,JAPAN

来源：

NEPHRON | 1995年 / 69卷 / 04期

关键词：

PODOCALYXIN; URINARY; GLOMERULAR EPITHELIAL CELL INJURY; MONOCLONAL ANTIBODIES; IMMUNOFLUORESCENCE;

D O I：

10.1159/000188509

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 [临床医学]; 100201 [内科学];

摘要：

Immunofluorescent study of urine sediments was performed to detect glomerular epithelial cell injuries using specific monoclonal antibody against podocalyxin, a glycoprotein that is prominently expressed on glomerular epithelial cells. Three kinds of structures (casts, granules and cells) were stained in urine sediments from various glomerular diseases. The presence of podocalyxin in urines was confirmed by absorption test, immunoelectron microscopy and Western blotting. Podocalyxin was detected in the urinary sediments of patients with various forms of glomerulonephritis, particularly those with acute onset, The amount of their urinary excretion apparently indicates the degree of the glomerular epithelial cell injuries in glomerular diseases.

引用

页码：397 / 403

页数：7

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