TRANSCRIPTION FACTOR NF-KAPPA-B IS ACTIVATED IN MICROGLIA DURING EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS

被引:115
作者
KALTSCHMIDT, C
KALTSCHMIDT, B
LANNESVIEIRA, J
KREUTZBERG, GW
WEKERLE, H
BAEUERLE, PA
GEHRMANN, J
机构
[1] MAX PLANCK INST PSYCHIAT,D-82152 MARTINSRIED,GERMANY
[2] UNIV ZURICH HOSP,INST NEUROPATHOL,DEPT PATHOL,CH-8091 ZURICH,SWITZERLAND
关键词
TRANSCRIPTION FACTOR NF-KAPPA-B; CENTRAL NERVOUS SYSTEM INFLAMMATION; EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS; ASTROCYTES; MICROGLIA;
D O I
10.1016/0165-5728(94)90151-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
NF-kappa B is an inducible transcription factor involved in the induction of multiple genes during inflammatory processes. So far the information pertaining to the role of NF-kappa B in autoimmune processes has been restricted to in vitro analysis. To further characterize the role of NF-kappa B in vivo, the involvement of NF-kappa B has been studied by immunocytochemistry in T cell-mediated autoimmune encephalomyelitis (EAE) of the Lewis rat. In non-diseased animals, immunoreactivity for the DNA-binding subunit p50 and for the DNA-binding and transactivating subunit p65 was low and restricted to the surface of small to medium-sized blood vessels. Strong immunoreactivities for p50 and p65 were detected at the peak of clinical disease. At the recovery stage of EAE, p50 and p65 immunoreactivities had declined to base line levels. Within the resident glial cell population, p50 and p65-immunoreactive cells were identified as OX-42-positive microglia. GFAP-positive astrocytes did not show significant p50 or p65 immunoreactivity. In the core and the vicinity of perivascular inflammatory lesions, both ED-1-positive macrophages and W3/13-positive T lymphocytes and monocytes were strongly immunoreactive for NF-kappa B. Our data suggest a crucial involvement of the transcription factor NF-kappa B in autoimmune diseases of the central nervous system. Furthermore, NF-kappa B appears as a useful marker for inflammatory processes in vivo.
引用
收藏
页码:99 / 106
页数:8
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