IDENTIFICATION OF A PHOTOCHEMICALLY INACTIVE PHEOPHYTIN MOLECULE IN THE SPINACH D-1-D-2-CYT B(559) COMPLEX

被引:35
作者
MIMURO, M
TOMO, T
NISHIMURA, Y
YAMAZAKI, I
SATOH, K
机构
[1] NATL INST BASIC BIOL,DIV BIOL REGULAT,OKAZAKI,AICHI 444,JAPAN
[2] OKAYAMA UNIV,FAC SCI,DEPT BIOL,OKAYAMA 700,JAPAN
[3] HOKKAIDO UNIV,FAC ENGN,DEPT CHEM PROC ENGN,SAPPORO,HOKKAIDO 060,JAPAN
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1995年 / 1232卷 / 1-2期
基金
日本学术振兴会;
关键词
FLUORESCENCE; PHEOPHYTIN; PHOTOSYNTHESIS; REACTION CENTER II; SPINACH;
D O I
10.1016/0005-2728(95)00116-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A photochemically inactive pheophytin (Pheo) a molecule in the spinach D-2-D-2-cyt b(559) complex, which contains approximately 6 chlorophyll (Chl) a molecules and two beta-carotene molecules for every two Pheo a molecules, was identified by fluorescence excitation spectroscopy at 77K, and confirmed by absorption, linear dichroism (LD), and magnetic circular dichroism spectra, When the emission was monitored at 740 nm, photochemically active Pheo a was detected as peaks at 418, 513, 533, and 681 nm, as described in previous reports. However, when the emission was monitored at 665 nm, two bands were observed at 414 and 537 nm; these bands were insensitive to photochemical reduction of the primary acceptor and thus appear to be due to photochemically inactive Pheo a. The Qy band of this Pheo a molecule at 77K was estimated to be at 671 +/- 1 nm, based on the magnitude of the band-shift in photochemically active Pheo a. Two beta-carotene molecules in the complex were also discriminated by fluorescence excitation and LD spectra. These observations were discussed in the light of current understanding of the molecular organization of pigments and the relationship of pigment arrangement to optical properties in the reaction center of photosystem II.
引用
收藏
页码:81 / 88
页数:8
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