A SIMPLE ASSAY FOR QUANTIFYING THE INDUCIBLE ADHERENCE OF NEUTROPHILS

被引：37

作者：

OEZ, S ^{[1
]}

WELTE, K ^{[1
]}

PLATZER, E ^{[1
]}

KALDEN, JR ^{[1
]}

机构：

[1] HANOVER MED SCH,KINDERKLIN,W-3000 HANOVER 61,GERMANY

来源：

IMMUNOBIOLOGY | 1990年 / 180卷 / 4-5期

关键词：

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; FCS; fetal calf serum; MTT; OD; optical density; phorbol; 12-myristate; 13-acetate; PMA; recombinant human granulocyte colony stimulating factor; recombinant human granulocyte-macrophage colony stimulating factor; recombinant human interferon gamma; recombinant human tumor necrosis factor-alpha; rhGCSF; rhGM-CSF; rhIFN γ; rhTNF-α;

D O I：

10.1016/S0171-2985(11)80294-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

By making use of the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) reducing ability of stimulated human neutrophils, we developed an alternative method to quantify the adherence of neutrophils in vitro. After discarding non-adherent neutrophils, the adherent cells were exposed to MTT solution containing 10 ng/ml phorbol 12-myristate -13-acetate (PMA). Subsequently, MTT reduced by this simultaneous stimulation was measured optically and used to calculate percent of adhesion. In these experiments, heat-inactivated autologous serum and tumor necrosis factor alpha (rhTNF-α) were observed to promote the adherence to polystyrene surfaces. In contrast, minimal or no effects on neutrophil adherence were achieved in case of treatment with native autologous serum, recombinant human granulocyte colony stimulating factor (rhG-CSF), recombinant human granulocyte-macrophage colony stimulating factor (rhGMCSF), or recombinant human interferon gamma (rhIFN-γ). © 1990, Gustav Fischer Verlag · Stuttgart · New York. All rights reserved.

引用

页码：308 / 315

页数：8

共 19 条

[1]

ANDERSON DC, 1986, J IMMUNOL, V137, P15

[2]

BAEHNER RL, 1976, BLOOD, V48, P309

[3] LEUKOCYTE ADHESION RECEPTORS ARE STORED IN PEROXIDASE-NEGATIVE GRANULES OF HUMAN-NEUTROPHILS [J].