DUAL EFFECT OF FLUORIDE ON PHOSPHOINOSITIDE METABOLISM IN RAT-BRAIN CORTEX - STIMULATION OF PHOSPHOLIPASE-C AND INHIBITION OF POLYPHOSPHOINOSITIDE SYNTHESIS

被引：16

作者：

CLARO, E

WALLACE, MA

FAIN, JN

机构：

来源：

BIOCHEMICAL JOURNAL | 1990年 / 268卷 / 03期

关键词：

D O I：

10.1042/bj2680733

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effects of fluoride, guanosine 5'-[γ-thio]triphosphate (GTP[S]) and carbachol on phospholipase C and polyphosphoinositide synthesis. The experimental system consisted of membranes from rat brain cortex, with exogenous [3H]phosphatidylinositol ([3H]PtdIns) as substrate. In such systems, we have not found evidence to support carbachol and/or GTP[S] stimulation of PtdIns phosphorylation. Fluoride inhibited synthesis of PtdIns4P and PtdIns(4,5)P2 from PtdIns. Consequently, under conditions where breakdown of polyphosphoinositides by phospholipase C was dependent on PtdIns kinase activity, fluoride inhibited activation by GTP[S] plus carbachol of phospholipase C. When conditions allowed direct breakdown of PtdIns and precluded PtdIns kinase activity, the stimulatory effects of fluoride and GTP[S] plus carbachol on phospholipase C activity were additive.

引用

页码：733 / 737

页数：5

共 23 条

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