EFFECTS OF OXYGEN ON THE MEMBRANE-STRUCTURE AND THE METABOLISM OF LIPOPHILIC NITROXIDE IN RAT-LIVER MICROSOMES

被引：19

作者：

MIURA, Y ^{[1
]}

UTSUMI, H ^{[1
]}

KASHIWAGI, M ^{[1
]}

HAMADA, A ^{[1
]}

机构：

[1] SHOWA UNIV,SCH PHARMACEUT SCI,DEPT HLTH CHEM,SHINAGAWA KU,TOKYO 142,JAPAN

来源：

JOURNAL OF BIOCHEMISTRY | 1990年 / 108卷 / 04期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a123233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of oxygen on the metabolism of lipophilic nitroxide and the membrane structure of microsomes were investigated with an ESR spectrometer equipped with a hand-made gas-permeable sampling tube. The half life of gas change in the membrane was confirmed to be 48 s by the line-width of the ESR signal. The signal intensity of lipophilic nitroxide in microsomal membranes decreased with NADPH under N2 and increased under O2. The change of signal intensity was reversible, suggesting that lipophilic nitroxide can be used as an indicator of the redox state in membranes for in vivo ESR imaging. © 1990 Copyright, 1990 by the Journal of Biochemistry.

引用

页码：516 / 518

页数：3

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