SALT AND TEMPERATURE-DEPENDENT CONFORMATION CHANGES IN SPECTRIN FROM HUMAN-ERYTHROCYTE MEMBRANES

被引:40
作者
RALSTON, GB
DUNBAR, JC
机构
[1] Department of Biochemistry, The University of Sydney, Sydney
关键词
(Erythrocyte membrane); Circular dichroism; Conformation change; Optical rotatory dispersion; Spectrin;
D O I
10.1016/0005-2795(79)90083-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
ORD and CD measurements of spectrin, in both the dimer and tetramer association state, indicate a high proportion of α-helix in this protein. At temperatures below 27°C and 0.1 M NaCl, the tetramer has an apparent helix content of 73% and the dimer, 68%. The conformation of both states is dependent on salt concentration and temperature. Low ionic strength solutions of spectrin display lowered sedimentation coefficients and a decreased apparent helix content, indicating perhaps a slight refolding and expansion of the molecule. In addition, spectrin in low ionic strength solutions undergoes a broad temperature-dependent transition spread from 20 to 50°C, while in the presence of salt the transition is sharp and centered on 49°C. The temperature-dependent changes in low ionic strength solutions appear to parallel the dissociation of tetramer to dimer. © 1979.
引用
收藏
页码:20 / 30
页数:11
相关论文
共 23 条
[1]  
BENNETT V, 1977, J BIOL CHEM, V252, P2753
[2]   CALORIMETRIC STUDIES OF STRUCTURAL TRANSITIONS OF HUMAN ERYTHROCYTE-MEMBRANE - INVOLVEMENT OF SPECTRIN IN A-TRANSITION [J].
BRANDTS, JF ;
ERICKSON, L ;
LYSKO, K ;
SCHWARTZ, AT ;
TAVERNA, RD .
BIOCHEMISTRY, 1977, 16 (15) :3450-3454
[3]   DETERMINATION OF SECONDARY STRUCTURES OF PROTEINS BY CIRCULAR-DICHROISM AND OPTICAL ROTATORY DISPERSION [J].
CHEN, YH ;
YANG, JT ;
MARTINEZ, HM .
BIOCHEMISTRY, 1972, 11 (22) :4120-+
[4]   INCORPORATION OF P-32 INTO SPECTRIN AGGREGATES FOLLOWING INCUBATION OF ERYTHROCYTES IN P-32-LABELED INORGANIC-PHOSPHATE [J].
DUNBAR, JC ;
RALSTON, GB .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 510 (02) :283-291
[5]  
ELGSAETER A, 1978, BIOCHIM BIOPHYS ACTA, V536, P235, DOI 10.1016/0005-2795(78)90069-7
[6]   ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE [J].
FAIRBANKS, G ;
STECK, TL ;
WALLACH, DFH .
BIOCHEMISTRY, 1971, 10 (13) :2606-+
[7]   OPTICAL ROTATORY DISPERSION [J].
FASMAN, GD .
METHODS IN ENZYMOLOGY, 1963, 6 :928-957
[8]   PROPERTIES OF HIGH-MOLECULAR-WEIGHT PROTEIN (SPECTRIN) FROM HUMAN-ERYTHROCYTE MEMBRANES [J].
GRATZER, WB ;
BEAVEN, GH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 58 (02) :403-409
[9]  
JACKSON W, 1973, BIOCHEMISTRY, V12, P3362
[10]  
JIRGENSONS JB, 1973, OPTICAL ACTIVITY PRO