AMINO-ACID-ANALYSIS USING DERIVATIZATION WITH 9-FLUORENYLMETHYL CHLOROFORMATE AND REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：85

作者：

HAYNES, PA

SHEUMACK, D

KIBBY, J

REDMOND, JW

机构：

[1] CORBETT TECHNOL,LIDCOMBE,NSW 2141,AUSTRALIA

[2] ICI INSTRUMENTS,DINGLEY,VIC 3172,AUSTRALIA

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 540卷 / 1-2期

关键词：

D O I：

10.1016/S0021-9673(01)88807-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple procedure for the precolumn derivatisation of amino acids with 9-fluorenylmethyl chloroformate and a liquid chromatographic method for the separation of the derivatives with fluorimetric detection in the picomole range are reported. The procedure does not involve a solvent extraction and gives single, stable derivatives of the common protein amino acids. The method has been demonstrated on hydrolysates of proteins and peptides.

引用

页码：177 / 185

页数：9

共 27 条

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