IMMUNODETECTION OF ARTEMISININ IN ARTEMISIA-ANNUA CULTIVATED IN HYDROPONIC CONDITIONS

被引：34

作者：

JAZIRI, M

DIALLO, B

VANHAELEN, M

HOMES, J

YOSHIMATSU, K

SHIMOMURA, K

机构：

[1] NATL INST HYG SCI, TSUKUBA MED PLANT RES STN, 1 HACHIMANDAI, TSUKUBA, IBARAKI 305, JAPAN

[2] UNIV LIBRE BRUXELLES, PLANT MORPHOL LAB, B-1160 BRUSSELS, BELGIUM

[3] UNIV LIBRE BRUXELLES, PHARMACOGNOSY & BROMATOL LAB, B-1050 BRUSSELS, BELGIUM

来源：

PHYTOCHEMISTRY | 1993年 / 33卷 / 04期

关键词：

ARTEMISIA-ANNUA; ASTERACEAE; ELISA; HPLC; HYDROPONICS; SESQUITERPENOID; DEOXYARTEMISININ SYNTHESIS; ARTEMISININ DISTRIBUTION; ARTEMISININ;

D O I：

10.1016/0031-9422(93)85282-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly specific and sensitive ELISA method was developed for the detection and semi-quantitative determination of artemisinin and its structurally related compounds in crude extracts of Artemisia annua. The antibodies were raised in rabbits using a 10-succinyldihydroartemisinin-BSA conjugate as immunogen. The peroxide linkage in the artemisinin molecule was critical in determining the antibody specificity. The working range of the assay was from 0.02 to 10 ng per assay. The cross-reacting material in crude plant extracts was evaluated by chromatographic methods combined with the immunoassay method. The distribution of artemisinin equivalents in five-week-old A. annua plants cultivated in hydroponic conditions was investigated. The highest artemisinin equivalent content (1.12% dry wt) was found in the leaves of the upper parts of the plant.

引用

页码：821 / 826

页数：6

共 23 条

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