TOXOPLASMA-GONDII - ACTIVITY OF THE POLYETHER IONOPHOROUS ANTIBIOTIC NIGERICIN ON TACHYZOITES IN CELL-CULTURE

被引：11

作者：

COUZINET, S ^{[1
]}

DUBREMETZ, JF ^{[1
]}

DAVID, L ^{[1
]}

PRENSIER, G ^{[1
]}

机构：

[1] INSERM, U42, F-59651 VILLENEUVE DASCQ, FRANCE

来源：

EXPERIMENTAL PARASITOLOGY | 1994年 / 78卷 / 04期

关键词：

TOXOPLASMA GONDII; PROTOZOA; PARASITE; IONOPHOROUS ANTIBIOTICS; CELL CULTURE; IN VITRO; BIOLOGICAL ACTIVITY; IMMUNOFLUORESCENCE; ELECTRON MICROSCOPY;

D O I：

10.1006/expr.1994.1037

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Polyether ionophorous antibiotics are widely used prophylactically to prevent coccidiosis in livestock production. The study of the effects of the nigericin on tachyzoites of Toxoplasma gondii clearly demonstrated that very low concentrations of this ionophore (0.05 mu g/ml) were sufficient to inhibit strongly the penetration and totally inhibit the intracellular development of parasites. Both nigericin and epinigericin showed a similar activity against tachyzoite development. However, the activity of abierixicin was 50-fold lower. Such antibiotic concentrations did not seem to affect host cells. Immunofluorescence and electron microscopy showed important changes in the cytology of the antibiotic-treated parasites: they were vacuolated or swollen and were sometimes found burst open, having lost their original shape. The magnitude and the frequency of alterations rose as concentrations in ionophore increased, (C) 1994 Academic Press, Inc,

引用

页码：341 / 351

页数：11

共 25 条

[1] THE ION SELECTIVITY OF NONELECTROGENIC IONOPHORES MEASURED ON A BILAYER LIPID-MEMBRANE - NIGERICIN, MONENSIN, A23187 AND LASALOCID-A [J].