SIMPLE MEASUREMENT OF CAPTOPRIL IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

被引：25

作者：

KLEIN, J

COLIN, P

SCHERER, E

LEVY, M

KOREN, G

机构：

[1] HOSP SICK CHILDREN,DEPT PEDIAT,DIV CLIN PHARMACOL,555 UNIV AVE,TORONTO M5G 1X8,ONTARIO,CANADA

[2] HOSP SICK CHILDREN,RES INST,TORONTO M5G 1X8,ONTARIO,CANADA

[3] UNIV MONTREAL,FAC PHARM,MONTREAL H3C 3J7,QUEBEC,CANADA

[4] SQUIBB CANADA INC,DEPT PROD DEV,MONTREAL,QUEBEC,CANADA

来源：

THERAPEUTIC DRUG MONITORING | 1990年 / 12卷 / 01期

关键词：

Captopril; DDPM; High-performance liquid chromatography; Ultraviolet detection; ρ-bromophenacyl bromide;

D O I：

10.1097/00007691-199001000-00019

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Captopril is an orally active inhibitor of angiotensin-converting enzyme. A rapid, accurate, and sensitive high-performance chromatography (HPLC) method is described for measuring plasma concentrations of captopril. Captopril was stabilized by forming an adduct with p-bromophenacyl bromide. This adduct was measured by HPLC using a C-18 reverse-phase column and monitoring the column effluent by ultraviolet absorption at 260 nm. The method proved to be linear in the clinical range of 10–1,000 ng/ml. The plasma levels of captopril in a young patient given a small oral dose were determined by this method. © 1992 Raveen Press. Ltd., New York.

引用

页码：105 / 110

页数：6

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