PURIFICATION AND CHARACTERIZATION OF URACIL PHOSPHORIBOSYLTRANSFERASE FROM CRITHIDIA-LUCILIAE

被引：14

作者：

ASAI, T ^{[1
]}

LEE, CS ^{[1
]}

CHANDLER, A ^{[1
]}

OSULLIVAN, WJ ^{[1
]}

机构：

[1] UNIV NEW S WALES,SCH BIOCHEM,KENSINGTON,NSW 2033,AUSTRALIA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1990年 / 95卷 / 01期

关键词：

D O I：

10.1016/0305-0491(90)90264-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Uracil phosphoribosyltransferase (UPRTase) was purified 370-fold from the protozoan parasite, Crithidia luciliae. 2. 2. The enzyme was a dimer of mol. wt 80,000 and was highly specific for uracil. 3. 3. GTP, which is an activator of UPRTase from E. coli had a slight inhibitory effect on the parasite enzyme. 4. 4. The C. luciliae UPRTase demonstrated a broad specificity for activating divalent metal ions. © 1989.

引用

页码：159 / 163

页数：5

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