PURIFICATION OF POLIOVIRUS BY AFFINITY-CHROMATOGRAPHY

被引：7

作者：

BROWN, F ^{[1
]}

UNDERWOOD, BO ^{[1
]}

FANTES, KH ^{[1
]}

机构：

[1] WELLCOME RES LABS,BECKENHAM BR3 3BS,KENT,ENGLAND

来源：

JOURNAL OF MEDICAL VIROLOGY | 1979年 / 4卷 / 04期

关键词：

affinity chromatography; poliovirus purification;

D O I：

10.1002/jmv.1890040409

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Poliovirus type 1 (Mahoney strain) has been purified by retention on a Sepharose 4B‐antibody column and elution with 3M K thiocyanate. The virus was recovered in excellent yield and its purity was as high as that achieved by detergent treatment followed by sucrose gradient centrifugation. The column could be re‐used and its capacity was sufficiently high to make it a useful method for the purification of milligram quantities of the virus. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company

引用

页码：315 / 319

页数：5