RAUSCHER LEUKEMIA VIRUS-INDUCED TUMOR-ANTIGENS - COMPLETE SEPARATION FROM GP70, P-30 AND H-2

The possibility that some or all of the viral proteins, gp 70, p 30, and the histocompatibility antigen, H‐2, function as the tumor‐specific transplantation antigen (TSTA) of the R‐MuLV‐induced leukemia, RBL‐5, and also in the secondary in vitro induction of cytotoxic T lymphocytes (CTL), was investigated. The antigen was obtained by isolating the plasma membranes of RBL‐5 cells and solubilizing with sodium deoxycholate (DOC) followed by gel filtration chromatography. A fraction containing excellent tumor‐rejection activity but low amounts of gp 70, p 30 and H‐2 was chromatographed on goat anti‐gp 70 goat anti‐p 30 and sheep anti‐H‐2b immunoaffinity columns. The data obtained indicate that gp 70, p 30 or H‐2 do not function as TSTA of RBL‐5 leukemia, individually or as a complex. Similarly, the antigen responsible for the specific secondary induction of CTL in vitro is distinct from these three proteins. Copyright © 1979 Wiley‐Liss, Inc., A Wiley Company