SELECTIVE AND QUANTITATIVE ISOLATION AND DETERMINATION OF APOMORPHINE IN HUMAN PLASMA

被引：17

作者：

ESSINK, AWG

LOHUIS, CPGG

ELHORST, JTK

RUTTEN, WJ

机构：

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 570卷 / 02期

关键词：

D O I：

10.1016/0378-4347(91)80548-Q

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple extraction system for the selective and quantitative isolation of apomorphine from human plasma is described. Apomorphine and N-n-propylnorapomorphine were isolated by complex formation between a borate group and the diol group of the apomorphines in an alkaline medium, this in combination with ion-pair formation. The reproducibility and linearity of this extraction method combined with high-performance liquid chromatography with electrochemical detection is excellent. The absolute mean recovery of apomorphine was 100%, the recovery of N-n-propylnorapomorphine was 98%. The detection limit of apomorphine in human plasma in the described system is approximately 0.5 ng/ml.

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收藏

页码：419 / 424

页数：6

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