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MAPPING AND TRANSCRIPTIONAL PROPERTIES OF RT1 CLASS-II REGION GENES

被引：12
作者：
FUJII, H
KAKINUMA, M
YOSHIKI, T
NATORI, T
机构：
[1] PALM INST,N29 W4 2-1-215,SAPPORO 001,JAPAN
[2] HOKKAIDO UNIV,SCH MED,DEPT PATHOL 1,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN
[3] HOKKAIDO UNIV,INST IMMUNOL SCI,BACTERIAL INFECT SECT,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN
来源：
TRANSPLANTATION | 1991年 / 52卷 / 02期
关键词：
D O I：
10.1097/00007890-199108000-00035
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
The class II region of major histocompatibility complex of the rat. Rattus norvegicus (RT1) consists of RT1.B, RT1.D, and RT1.H subregions. The gene order around the H subregion was determined as RT1.A--H-beta-H-alpha--B by RFLP analysis of naturally occurring intra-RT1 recombinant rats with HLA DP probes. A unique recombinant strain, LEJ, was found to have its recombinational site between H-beta and H-alpha (RT1.A(u)H-beta(u)H-alpha(b)B(b)D(b)). Northern analysis of class II mRNAs showed that transcripts of RT1.D-alpha, RT1.D-beta, and RT1.B-alpha shared identical sizes among various strains of rats, but RT1.B-beta mRNA showed allele-specific size heterogeneities. Northern hybridization with HLA DP-alpha probes detected possible RT1.H-alpha transcripts. On the other hand, no clear signal of H-beta was observed. BDIX whose RT1.B products had not been identified was found to transcribe B-alpha and B-beta mRNAs.
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页码：369 / 373
页数：5
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