KILLING OF CELLS BY PERFORIN - RESISTANCE TO KILLING IS NOT DUE TO DIMINISHED BINDING OF PERFORIN TO THE CELL-MEMBRANE

被引:11
作者
JONES, J [1 ]
MORGAN, BP [1 ]
机构
[1] UNIV WALES COLL MED,DEPT MED BIOCHEM,CARDIFF CF4 4XN,S GLAM,WALES
基金
英国惠康基金;
关键词
D O I
10.1042/bj2800199
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Different cell types vary widely in their susceptibility to killing by the pore-forming cytolytic molecule perforin. In particular, the cells responsible for synthesis of perforin, i.e. cytotoxic T lymphocytes (CTL) and natural killer (NK) cells, are very resistant to cytolysis by this molecule. It has previously been suggested that resistance is due, at least in part, to diminished binding of perforin to these cells. The purpose of the present study was to compare binding of perforin to sensitive and resistant cell types. To this end, perforin was biosynthetically labelled prior to purification. The purified labelled protein was then utilized to obtain a direct measure of the amount of perforin bound to cells during attack. Resistant cells (CTL, neutrophils) bound at least as much perforin as did sensitive cells (K562, HL60 etc.), indicating that resistance to perforin involves mechanisms operating after binding of the lytic molecule.
引用
收藏
页码:199 / 204
页数:6
相关论文
共 26 条
[1]   MONOCLONAL-ANTIBODIES DEMONSTRATE PROTECTION OF POLYMORPHONUCLEAR LEUKOCYTES AGAINST COMPLEMENT ATTACK [J].
CAMPBELL, AK ;
MORGAN, BP .
NATURE, 1985, 317 (6033) :164-166
[2]   MECHANISM OF LYMPHOCYTE-MEDIATED CYTO-TOXICITY [J].
HENKART, PA .
ANNUAL REVIEW OF IMMUNOLOGY, 1985, 3 :31-58
[3]  
ISHIURA S, 1990, MOL IMMUNOL, V27, P803
[4]  
JIANG S, 1990, J IMMUNOL, V144, P998
[5]   RESISTANCE OF CYTOLYTIC LYMPHOCYTES TO PERFORIN-MEDIATED KILLING - LACK OF CORRELATION WITH COMPLEMENT-ASSOCIATED HOMOLOGOUS SPECIES RESTRICTION [J].
JIANG, S ;
PERSECHINI, PM ;
ZYCHLINSKY, A ;
LIU, CC ;
PERUSSIA, B ;
YOUNG, JDE .
JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 168 (06) :2207-2219
[6]  
JIANG SB, 1990, J IMMUNOL METHODS, V126, P29
[7]   REVERSIBLE CELL-DAMAGE BY T-CELL PERFORINS - CALCIUM INFLUX AND PROPIDIUM IODIDE UPTAKE INTO K562 CELLS IN THE ABSENCE OF LYSIS [J].
JONES, J ;
HALLETT, MB ;
MORGAN, BP .
BIOCHEMICAL JOURNAL, 1990, 267 (02) :303-307
[8]   PERFORIN, A PORE-FORMING PROTEIN DETECTABLE BY MONOCLONAL-ANTIBODIES, IS A FUNCTIONAL MARKER FOR KILLER-CELLS [J].
KAWASAKI, A ;
SHINKAI, Y ;
KUWANA, Y ;
FURUYA, A ;
IIGO, Y ;
HANAI, N ;
ITOH, S ;
YAGITA, H ;
OKUMURA, K .
INTERNATIONAL IMMUNOLOGY, 1990, 2 (07) :677-684
[9]   STRUCTURE AND FUNCTION OF HUMAN PERFORIN [J].
LICHTENHELD, MG ;
OLSEN, KJ ;
LU, P ;
LOWREY, DM ;
HAMEED, A ;
HENGARTNER, H ;
PODACK, ER .
NATURE, 1988, 335 (6189) :448-451
[10]   CLONING, ANALYSIS, AND EXPRESSION OF MURINE PERFORIN-1 CDNA, A COMPONENT OF CYTOLYTIC T-CELL GRANULES WITH HOMOLOGY TO COMPLEMENT COMPONENT-C9 [J].
LOWREY, DM ;
AEBISCHER, T ;
OLSEN, K ;
LICHTENHELD, M ;
RUPP, F ;
HENGARTNER, H ;
PODACK, ER .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (01) :247-251