C-CAM (CELL-CAM-105) IS A CALMODULIN BINDING-PROTEIN

被引：22

作者：

BLIKSTAD, I

WIKSTROM, T

AURIVILLIUS, M

OBRINK, B

机构：

[1] KAROLINSKA INST, MED NOBEL INST, DEPT MED CELL BIOL, BOX 60400, S-10401 STOCKHOLM 60, SWEDEN

[2] UNIV UPPSALA, CTR BIOMED, DEPT MED & PHYSIOL CHEM, S-75123 UPPSALA, SWEDEN

来源：

FEBS LETTERS | 1992年 / 302卷 / 01期

基金：

英国医学研究理事会;

关键词：

CELL ADHESION MOLECULE (CAM); CALMODULIN; CELL ADHESION; TRANSMEMBRANE SIGNALING;

D O I：

10.1016/0014-5793(92)80276-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

C-CAM (Cell-CAM 105) is a transmembrane cell adhesion molecule belonging to the immunoglobulin superfamily. It mediates intercellular adhesion of rat hepatocytes and occurs in various isoforms in several epithelia, vessel endothelia and leukocytes. We now report that purified liver C-CAM interacts specifically with calmodulin. Binding was observed both when I-125-labeled C-CAM was used in a dot-blot assay and when I-125-labeled calmodulin was used in a gel overlay assay. Experiments with protease-generated peptides indicated that calmodulin bound to the cytoplasmic domain of C-CAM. Analyses of whole liver membranes demonstrated that C-CAM is one of five major proteins that bind calmodulin in a calcium-dependent manner.

引用

页码：26 / 30

页数：5

共 21 条

[1] THE CELL-ADHESION MOLECULE CELL-CAM-105 IS AN ECTO-ATPASE AND A MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY [J].