BIOLOGICAL-ACTIVITIES OF RABBIT ANTIBODIES AGAINST SYNTHETIC HUMAN THYROTROPIN RECEPTOR PEPTIDES REPRESENTING THYROTROPIN BINDING REGIONS

被引：36

作者：

SAKATA, S ^{[1
]}

OGAWA, T ^{[1
]}

MATSUI, I ^{[1
]}

MANSHOURI, T ^{[1
]}

ATASSI, MZ ^{[1
]}

机构：

[1] BAYLOR COLL MED,DEPT BIOCHEM,HOUSTON,TX 77030

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 03期

关键词：

D O I：

10.1016/0006-291X(92)91884-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recently, we have shown that the thyrotropin (TSH) binding regions of human thyrotropin receptor (TSHR) reside in two areas within residues 12-44 and 308-344. Serial antisera were raised against four overlapping synthetic peptides representing these two regions of TSHR (peptides 12-30, 24-44, 308-328, and 324-344) and were investigated for their ability to stimulate or block the cultured porcine thyroid cells. In addition, serum concentrations of triiodothyronine (T3) and thyroxine (T4) in serial sera obtained from each rabbit were examined. It was shown that residues of 12-30 and 324-344 of TSHR, respectively, are the site (at least a part of the site) where stimulating (TSAb) and blocking type (TSBAb) immunoglobulins are directed. © 1992.

引用

页码：1369 / 1375

页数：7

共 24 条

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