EXTRACELLULAR 2-CHLOROADENOSINE AND ATP STIMULATE VOLUME-SENSITIVE CL- CURRENT AND CALCIUM MOBILIZATION IN HUMAN TRACHEAL 9HTEO- CELLS

被引：30

作者：

GALIETTA, LJV

RASOLA, A

RUGOLO, M

ZOTTINI, M

MASTROCOLA, T

GRUENERT, DC

ROMEO, G

机构：

[1] UNIV BOLOGNA,DIPARTIMENTO BIOL ES,I-40126 BOLOGNA,ITALY

[2] UNIV CALIF SAN FRANCISCO,CARDIOVASC & CANC RES INST,DEPT LAB MED,SAN FRANCISCO,CA 94143

来源：

FEBS LETTERS | 1992年 / 304卷 / 01期

关键词：

AIRWAY EPITHELIUM; CL-; CURRENT; CELL VOLUME; 2-CHLOROADENOSINE; ATP; INTRACELLULAR CA2+;

D O I：

10.1016/0014-5793(92)80589-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The perforated-patch whole-cell technique was used to record membrane currents in epithelial cells (9HTEo-) obtained from the human tracheal epithelium. Extracellular application of 2-chloroadenosine and ATP (0.01-100-mu-M) caused activation of Cl- currents similar to those regulated by cell volume in airway and intestinal cells. This response was inhibited by increasing extracellular osmolality, by omission of extracellular Ca2+, or by the addition of the A2 adenosine receptor antagonist 3,7-dimethyl-1-propargylxanthine (DMPX). Fluorimetric measurements with fura-2 reveal that 2-chloroadenosine and ATP elicited both a Ca2+ influx through the plasma membrane and a release from intracellular stores.

引用

页码：61 / 65

页数：5

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