DETECTION OF LEGIONELLA SPP IN COOLING-TOWER WATER BY THE POLYMERASE CHAIN-REACTION METHOD

被引：53

作者：

KOIDE, M ^{[1
]}

SAITO, A ^{[1
]}

KUSANO, N ^{[1
]}

HIGA, F ^{[1
]}

机构：

[1] UNIV RYUKYUS,FAC MED,DEPT INTERNAL MED 1,207 UEHARA,NISIHARA CHO,OKINAWA 90301,JAPAN

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1993年 / 59卷 / 06期

关键词：

D O I：

10.1128/AEM.59.6.1943-1946.1993

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.

引用

页码：1943 / 1946

页数：4

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