ISOELECTRIC FOCUSING OF SERUM PROTEINS IN ACRYLAMIDE GELS FOLLOWED BY ELECTROPHORESIS

被引:86
作者
DALE, G
LATNER, AL
机构
[1] University Department of Clinical Biochemistry, Royal Victoria Infirmary, Newcastle upon Tyne
关键词
D O I
10.1016/0009-8981(69)90141-7
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
A technique is described in which isoelectric focusing in acrylamide gel is followed by electrophoresis in the same medium as a two-dimensional procedure. Normal sera examined in this way have given recognisable protein maps. Sera in disease states show alterations in the pattern. The method has been applied to myelomatosis and cirrhosis and appears capable of separating IgA from IgG. The technique can be applied to other biological fluids including urine and cerebrospinal fluid. © 1968.
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页码:61 / &
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