K-CURRENT MEDIATION OF PROLACTIN-INDUCED PROLIFERATION OF MALIGNANT (NB2) LYMPHOCYTES

被引：37

作者：

WANG, YF ^{[1
]}

JIA, HJ ^{[1
]}

WALKER, AM ^{[1
]}

CUKIERMAN, S ^{[1
]}

机构：

[1] UNIV CALIF RIVERSIDE, DIV BIOMED SCI, RIVERSIDE, CA 92521 USA

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 1992年 / 152卷 / 01期

关键词：

D O I：

10.1002/jcp.1041520123

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The effects of different concentrations of various K-current blockers on prolactin-induced proliferation and membrane K-currents in malignant lymphocytes (Nb2 cells) were investigated. Membrane currents were measured with the whole cell patch-clamp technique, and lymphocyte density was quantified by both spectrophotometric and conventional methods. K-current blockers tested (quinidine, 4-aminopyridine, barium, and tetraethylammonium) exhibited similar rank order potency for K-current block and inhibition of prolactin-induced proliferation of malignant lymphocytes. Because Nb2 cells proliferate independently of a transmembrane Ca-influx, these results suggest that K-currents per se rather than K-current modulation of Ca-influx is an essential event for lymphocyte proliferation.

引用

页码：185 / 189

页数：5

共 34 条

[1] THE T11-GLYCOPROTEIN IS FUNCTIONALLY LINKED TO A CALCIUM-CHANNEL IN PRECURSOR AND MATURE T-LINEAGE CELLS [J].

ALCOVER, A ;

WEISS, MJ ;

DALEY, JF ;

REINHERZ, EL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (08) :2614-2618

[2]

AMIGORENA S, 1990, J IMMUNOL, V144, P2038

[3]

BRENT LH, 1990, J IMMUNOL, V145, P2381

[4] PROLACTIN STIMULATION OF ORNITHINE DECARBOXYLASE AND MITOGENESIS IN NB-2 NODE LYMPHOMA-CELLS - THE ROLE OF PROTEIN-KINASE-C AND CALCIUM MOBILIZATION [J].

BUCKLEY, AR ;

MONTGOMERY, DW ;

KIBLER, R ;

PUTNAM, CW ;

ZUKOSKI, CF ;

GOUT, PW ;

BEER, CT ;

RUSSELL, DH .

IMMUNOPHARMACOLOGY, 1986, 12 (01) :37-51

[5] A VOLTAGE-GATED POTASSIUM CHANNEL IN HUMAN LYMPHOCYTES-T [J].

CAHALAN, MD ;

CHANDY, KG ;

DECOURSEY, TE ;

GUPTA, S .

JOURNAL OF PHYSIOLOGY-LONDON, 1985, 358 (JAN) :197-237

[6] PROTEIN-KINASE ACTIVITY CLOSELY ASSOCIATED WITH A RECONSTITUTED CALCIUM-ACTIVATED POTASSIUM CHANNEL [J].

CHUNG, SK ;

REINHART, PH ;

MARTIN, BL ;

BRAUTIGAN, D ;

LEVITAN, IB .

SCIENCE, 1991, 253 (5019) :560-562

[7] STIMULATION OF GROWTH OF NB2 LYMPHOMA-CELLS BY INTERLEUKIN-2 IN SERUM-FREE AND SERUM-CONTAINING MEDIA [J].

CROZE, F ;

WALKER, A ;

FRIESEN, HG .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1988, 55 (2-3) :253-259

[8]

CUKIERMAN S, 1992, J MEMBRANE BIOL, V126, P147

[9] VOLTAGE-GATED K+ CHANNELS IN HUMAN LYMPHOCYTE-T - A ROLE IN MITOGENESIS [J].

DECOURSEY, TE ;

CHANDY, KG ;

GUPTA, S ;

CAHALAN, MD .

NATURE, 1984, 307 (5950) :465-468

[10] VOLTAGE-GATED POTASSIUM CONDUCTANCE IN HUMAN LYMPHOCYTES-T STIMULATED WITH PHORBOL ESTER [J].

DEUTSCH, C ;

KRAUSE, D ;

LEE, SC .

JOURNAL OF PHYSIOLOGY-LONDON, 1986, 372 :405-423

← 1 2 3 4 →