RFLP analysis of genetic variation in North American populations of the fall armyworm moth Spodoptera frugiperda (Lepidoptera: Noctuidae)

被引:48
作者
Lu, Yang-Jiang [1 ]
Adang, M. J. [1 ]
Isenhour, D. J. [1 ]
Kochert, G. D. [2 ]
机构
[1] Univ Georgia, Dept Entomol, Athens, GA 30602 USA
[2] Univ Georgia, Dept Bot, Athens, GA 30602 USA
关键词
fall armyworm; genetic variation; Lepidoptera; molecular markers; Noctuidae; RFLP; Spodoptera frugiperda;
D O I
10.1111/j.1365-294X.1992.tb00178.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A high level of genetic variability was detected in North American fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), populations by restriction fragment length polymorphism (RFLP) analysis of genomic DNA. In nearly all cases individual larvae could be differentiated using 22 probe-enzyme combinations. Laboratory colonies formed distinct groups based on RFLP analysis. Individuals from a colony of the previously determined 'rice strain' formed a. group showing obvious deviation in RFLP patterns from the other five populations, which included a population from a 'corn strain' colony. The results were consistent with previous studies using allozymes which indicated that there are two genetically differentiated forms of the fall armyworm. Diagnostic markers were also found that distinguish these two groups.
引用
收藏
页码:199 / 207
页数:9
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