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INFLUENCE OF RAT STRAIN ON P-GLYCOPROTEIN EXPRESSION IN CULTURED-HEPATOCYTES

被引：5
作者：
CHIELI, E
ROMITI, N
CERVELLI, F
PAOLICCHI, A
TONGIANI, R
机构：
[1] Dipartimento di Biomedicina, Sezione di Patologia Generale, Scuola Medica, Pisa, I-66126
来源：
CELL BIOLOGY AND TOXICOLOGY | 1994年 / 10卷 / 03期
关键词：
P-GLYCOPROTEIN; CULTURED HEPATOCYTES; RAT STRAIN; RHODAMINE-123;
D O I：
10.1007/BF00757559
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The amount and activity of the multi-drug transporter P-glycoprotein (Pgp) have been measured in cultured hepatocytes derived from different rat strains. A marked increase in Pgp, as revealed by Western blotting, occurred 48 h after seeding in hepatocytes from Sprague-Dawley, Wistar and Fischer 344 rats, the last showing the highest value. The addition of dexamethasone (DEX) to culture medium delayed Pgp overexpression in all the strains, proportionally to the protein amount in the absence of hormone. The R-123 functional test for Pgp showed that Fischer 344 hepatocytes had the lowest ability to extrude the fluorescent dye as compared with Sprague-Dawley or Wistar rats. These results suggest that the Fischer 344 rat is more prone than other strains to culture stressing conditions, leading to an overexpression of Pgp that is not necessarily functional.
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页码：163 / 166
页数：4
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