NONSTEROIDAL ANTIINFLAMMATORY DRUGS INHIBIT GASTRIC PEROXIDASE-ACTIVITY

被引：46

作者：

BANERJEE, RK

机构：

[1] Department of Physiology, Indian Institute of Chemical Biology, Calcutta

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1034卷 / 03期

关键词：

Enzyme inhibition; Gastric peroxidase; Nonsteroidal anti-inflammatory drug;

D O I：

10.1016/0304-4165(90)90050-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The peroxidase activity of the mitochondrial fraction of rat gastric mucosa was inhibited with various nonsteroidal anti-inflammatory drugs (NSAIDs) in vitro. Indomethacin was found to be more effective than phenylbutazone (PB) or acetylsalicylic acid (ASA). Mouse gastric peroxidase was also very sensitive to indomethacin inhibition. Indomethacin has no significant effect on submaxillary gland peroxidase activity of either of the species studied. Purified rat gastric peroxidase activity was inhibited 75% with 0.15 mM indomethacin showing half-maximal inhibition at 0.04 mM. The inhibition could be withdrawn by increasing the concentration of iodide but not by H2O2. NSAIDs inhibit gastric peroxidase activity more effectively at acid pH (pH 5.2) than at neutral pH. Spectral studies showed a bathochromic shift of the Soret band of the enzyme with indomethacin indicating its interaction at or near the heme part of the enzyme. © 1990.

引用

页码：275 / 280

页数：6

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